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- Title
Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF<sup>59</sup>-U2AF<sup>23</sup>.
- Authors
Tao Huang; Vilardell, Josep; Query, Charles C.
- Abstract
We have initiated a biochemical analysis of splicing complexes in extracts from the fission yeast Schizosaccharomyces pombe. Extracts of S.pombe contain high levels of the spliceosome-like U2/5/6 tri-snRNP, which dissociates into mono-snRNPs in the presence of ATP, and supports binding of U2 snRNP to the 3' end of introns, yielding a weak ATP-independent E complex and the stable ATP-dependent complex A. The requirements for S.pombe complex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U2AF) are analogous to those of mammalian complex A. The S.pombe SF1/BBP, U2AF59 and U2AF23 are tightly associated in a novel complex that is required for complex A formation. This pre-formed SF1-U2AF59-U2AF23 complex may represent a streamlined mechanism for recognition of the branch site, pyrimidine tract and 3' splice site at the 3' end of introns.
- Publication
EMBO Journal, 2002, Vol 21, Issue 20, p5516
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1093/emboj/cdf555