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- Title
Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.
- Authors
Dichtl, Bernhard; Blank, Diana; Sadowski, Martin; Hübner, Wolfgang; Weiser, Stefan; Keller, Walter
- Abstract
RNA polymerase II (pol II) transcription termination requires co-transcriptional recognition of a functional polyadenylation signal, but the molecular mechanisms that transduce this signal to pol II remain unclear. We show that Yhh1p/Cft1p, the yeast homologue of the mammalian AAUAAA interacting protein CPSF 160, is an RNA-binding protein and provide evidence that it participates in poly(A) site recognition. Interestingly, RNA binding is mediated by a central domain composed of predicted beta-propeller-forming repeats, which occurs in proteins of diverse cellular functions. We also found that Yhh1p/Cft1p bound specifically to the phosphorylated C-terminal domain (CTD) of pol II in vitro and in a two-hybrid test in vivo. Furthermore, transcriptional run-on analysis demonstrated that yhh1 mutants were defective in transcription termination, suggesting that Yhh1p/Cft1p functions in the coupling of transcription and 3'-end formation. We propose that direct interactions of Yhh1p/Cft1p with both the RNA transcript and the CTD are required to communicate poly(A) site recognition to elongating pol II to initiate transcription termination.
- Publication
The EMBO journal, 2002, Vol 21, Issue 15, p4125
- ISSN
0261-4189
- Publication type
Journal Article
- DOI
10.1093/emboj/cdf390