We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation.
- Authors
Quiping Cao; Richter, Joel D.
- Abstract
Cytoplasmic polyadenylation stimulates the translation of several dormant mRNAs during oocyte maturation in Xenopus. Polyadenylation is regulated by the cytoplasmic polyadenylation element (CPE), a cis-acting element in the 3'-untranslated region of responding mRNAs, and its associated factor CPEB. CPEB also binds maskin, a protein that in turn interacts with eIF4E, the cap-binding factor. Here, we report that based on antibody and mRNA reporter injection assays, maskin prevents oocyte maturation and the translation of the CPE-containing cyclin Bi mRNA by blocking the association of eIF4G with eIF4E. Dissociation of the maskin-eIF4E complex is essential for cyclin Bi mRNA translational activation, and requires not only cytoplasmic polyadenylation, but also the poly(A)-binding protein. These results suggest a molecular mechanism by which CPE-containing mRNA is activated in early development.
- Publication
EMBO Journal, 2002, Vol 21, Issue 14, p3852
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1093/emboj/cdf353