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- Title
Ca<sup>2+</sup> dynamics in the lumen of the endoplasmic reticulum in sensory neurons: direct visualization of Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release triggered by physiological Ca<sup>2+</sup> entry.
- Authors
Solovyova, N.; Veselovsky, N.; Toescu, E. C.; Verkhratsky, A.
- Abstract
In cultured rat dorsal root ganglia neurons, we measured membrane currents, using the patch-clamp whole-cell technique, and the concentrations of free Ca2+ in the cytosot ([Ca2+]i) and in the lumen of the endoptasmic reticulum (ER) ([Ca2+]L), using high- (Fluo-3) and low- (Mag-Fura-2) affinity Ca2+-sensitive fluorescent probes and video imaging. Resting [Ca2+]L concentration varied between 60 and 270 μM. Activation of ryanodine receptors by caffeine triggered a rapid fall in [Ca2+]L levels, which amounted to only 40-50% of the resting [Ca2+]L value. Using electrophysiological depolarization, we directly demonstrate the process of Ca2+-induced Ca2+ release triggered by Ca2+ entry through voltage-gated Ca2+ channels. The amplitude of Ca2+ release from the ER lumen was linearly dependent on ICa.
- Publication
EMBO Journal, 2002, Vol 21, Issue 4, p622
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1093/emboj/21.4.622