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- Title
ß-arrestin- and c-Src-dependent degradation of G-protein-coupled receptor kinase 2.
- Authors
Penela, Petronila; Elorza, Ana; Sarnago, Susana; Mayor Jr, Federico
- Abstract
G-protein-coupled receptor kinase 2 (GRK2) plays a key role in the regulation of G-protein-coupled receptors (GPCRs). GRK2 expression is altered in several pathological conditions, but the molecular mechanisms that modulate GRK2 cellular levels are largely unknown. We recently have described that GRK2 is degraded rapidly by the proteasome pathway. This process is enhanced by GPCR stimulation and is severely impaired in a GRK2 mutant that tacks kinase activity (GRK2-K220R). In this report, we find that β-arrestin function and Src-mediated phosphorylation of GRK2 are critically involved in GRK2 proteolysis. Overexpression of β-arrestin triggers GRK2-K220R degradation based on its ability to recruit c-Src, since this effect is not observed with β-arrestin mutants that display an impaired c-Src interaction. The presence of an inactive c-Src mutant or of tyrosine kinase inhibitors strongly inhibits co-transfected or endogenous GRK2 turnover, respectively, and a GRK2 mutant with impaired phosphorylation by c-Src shows a markedly retarded degradation. This pathway for the modulation of GRK2 protein stability puts forward a new feedback mechanism for regulating GRK2 levels and GPCR signaling.
- Publication
EMBO Journal, 2001, Vol 20, Issue 18, p5129
- ISSN
0261-4189
- Publication type
Academic Journal
- DOI
10.1093/emboj/20.18.5129