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- Title
GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28.
- Authors
Sagiv, Y; Legesse-Miller, A; Porat, A; Elazar, Z
- Abstract
Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.
- Publication
The EMBO journal, 2000, Vol 19, Issue 7, p1494
- ISSN
0261-4189
- Publication type
Journal Article
- DOI
10.1093/emboj/19.7.1494