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- Title
TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins.
- Authors
Kanai, F; Marignani, P A; Sarbassova, D; Yagi, R; Hall, R A; Donowitz, M; Hisaminato, A; Fujiwara, T; Ito, Y; Cantley, L C; Yaffe, M B
- Abstract
The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. By screening in vitro translated cDNA pools for the ability to bind 14-3-3, we identified a novel transcriptional co-activator, TAZ (transcriptional co-activator with PDZ-binding motif) as a 14-3-3-binding molecule. TAZ shares homology with Yes-associated protein (YAP), contains a WW domain and functions as a transcriptional co-activator by binding to the PPXY motif present on transcription factors. 14-3-3 binding requires TAZ phosphorylation on a single serine residue, resulting in the inhibition of TAZ transcriptional co-activation through 14-3-3-mediated nuclear export. The C-terminus of TAZ contains a highly conserved PDZ-binding motif that localizes TAZ into discrete nuclear foci and is essential for TAZ-stimulated gene transcription. TAZ uses this same motif to bind the PDZ domain-containing protein NHERF-2, a molecule that tethers plasma membrane ion channels and receptors to cytoskeletal actin. TAZ may link events at the plasma membrane and cytoskeleton to nuclear transcription in a manner that can be regulated by 14-3-3.
- Publication
The EMBO journal, 2000, Vol 19, Issue 24, p6778
- ISSN
0261-4189
- Publication type
Journal Article
- DOI
10.1093/emboj/19.24.6778