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- Title
A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA.
- Authors
Allen, M D; Yamasaki, K; Ohme-Takagi, M; Tateno, M; Suzuki, M
- Abstract
The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.
- Publication
The EMBO journal, 1998, Vol 17, Issue 18, p5484
- ISSN
0261-4189
- Publication type
Journal Article
- DOI
10.1093/emboj/17.18.5484