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- Title
Diverse transcriptional initiation revealed by fine, large-scale mapping of mRNA start sites.
- Authors
Suzuki, Y; Taira, H; Tsunoda, T; Mizushima-Sugano, J; Sese, J; Hata, H; Ota, T; Isogai, T; Tanaka, T; Morishita, S; Okubo, K; Sakaki, Y; Nakamura, Y; Suyama, A; Sugano, S
- Abstract
Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The 'oligo-capping' method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo-capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5'-end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.
- Publication
EMBO reports, 2001, Vol 2, Issue 5, p388
- ISSN
1469-221X
- Publication type
Journal Article
- DOI
10.1093/embo-reports/kve085