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- Title
Structural analysis and complete physical map of Arabidopsis thaliana chromosome 5 including centromeric and telomeric regions.
- Authors
Kotani, H; Hosouchi, T; Tsuruoka, H
- Abstract
Previously, we have reported a fine physical map of Arabidopsis thaliana chromosome 5, except for the centromeric and telomeric regions, by ordering clones from YAC, P1, TAC, and BAC libraries of the genome consisting of the two contigs of upper arm and lower arm, 11.6 M bases and 14.2 M bases, respectively. Here, the remaining centromeric and telomeric regions of chromosome 5 are completely characterized by the ordering of clones and PCR amplifications. Chromosome 5 of Arabidopsis thaliana ecotype Columbia is about 28.4 M bases long. The centromeric region is estimated at about 2 M bases long between two 5S-rDNA clusters. The 180-bp repeat region mainly consists of blocks of 180-bp tandem family and various type retroelements dispersed over a 500-kb region. The telomeric regions of chromosome 5 are characterized by PCR cloning, sequencing and hybridization. The telomere repeats at both ends are about 2.5-kb long and interestingly, telomere-associated repeats (approximately 700 bp) are found near both ends of chromosome 5.
- Publication
DNA research : an international journal for rapid publication of reports on genes and genomes, 1999, Vol 6, Issue 6, p381
- ISSN
1340-2838
- Publication type
Journal Article
- DOI
10.1093/dnares/6.6.381