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- Title
A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.
- Authors
Itoh, T; Aiba, H; Baba, T; Hayashi, K; Inada, T; Isono, K; Kasai, H; Kimura, S; Kitakawa, M; Kitagawa, M; Makino, K; Miki, T; Mizobuchi, K; Mori, H; Mori, T; Motomura, K; Nakade, S; Nakamura, Y; Nashimoto, H; Nishio, Y; Oshima, T; Saito, N; Sampei, G; Seki, Y; Horiuchi, T
- Abstract
The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element.
- Publication
DNA research : an international journal for rapid publication of reports on genes and genomes, 1996, Vol 3, Issue 6, p379
- ISSN
1340-2838
- Publication type
Journal Article
- DOI
10.1093/dnares/3.6.379