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- Title
Evidence of anti-benzo[a]pyrene diolepoxide-DNA adduct formation in human colon mucosa.
- Authors
Alexandrov, K; Rojas, M; Kadlubar, F F; Lang, N P; Bartsch, H
- Abstract
As risk factors for colorectal cancer include consumption of foods potentially contaminated with polycyclic aromatic hydrocarbons (PAHs), the level of (+)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(+)-anti-BPDE] bound to DNA of human colon mucosa samples was quantified by a sensitive and specific HPLC/fluorescence method (Alexandrov et al., Cancer Res. 51, 6248-6253, 1992). (+)-anti-BPDE-DNA adducts were detected in four out of seven colon mucosa samples but not in any of 11 human pancreas samples from smokers and non-smokers. Adduct levels in human colon varied between 0.2 and 1.0 (+)-anti-BPDE-DNA adducts/10(8) nucleotides. Our results provide evidence that: (i) the DNA in human colon cells can be damaged by benzo[a]pyrene, possibly derived from diet and/or tobacco smoke; (ii) DNA adduct formation in human colon epithelium proceeds via the diol epoxide pathway; (iii) benzo[a]pyrene and other PAHs could play a role in the etiology of human colorectal cancer.
- Publication
Carcinogenesis, 1996, Vol 17, Issue 9, p2081
- ISSN
0143-3334
- Publication type
Journal Article
- DOI
10.1093/carcin/17.9.2081