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- Title
RNA-Seq gene expression estimation with read mapping uncertainty.
- Authors
Bo Li; Ruotti, Victor; Stewart, Ron M.; Thomson, James A.; Dewey, Colin N.
- Abstract
Motivation: RNA-Seq is a promising new technology for accurately measuring gene expression levels. Expression estimation with RNA-Seq requires the mapping of relatively short sequencing reads to a reference genome or transcript set. Because reads are generally shorter than transcripts from which they are derived, a single read may map to multiple genes and isoforms, complicating expression analyses. Previous computational methods either discard reads that map to multiple locations or allocate them to genes heuristically.
- Publication
Bioinformatics, 2010, Vol 26, Issue 4, p493
- ISSN
1367-4803
- Publication type
Academic Journal
- DOI
10.1093/bioinformatics/btp692