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- Title
Reliable prediction of protein thermostability change upon double mutation from amino acid sequence.
- Authors
Liang-Tsung Huang; Gromiha, M. Michael
- Abstract
Summary: The accurate prediction of protein stability change upon mutation is one of the important issues for protein design. In this work, we have focused on the stability change of double mutations and systematically analyzed the wild-type and mutant residues, patterns in amino acid sequence and locations of mutants. Based on the sequence information of wild-type, mutant and three neighboring residues, we have presented a weighted decision table method (WET) for predicting the stability changes of 180 double mutants obtained from thermal (ΔΔG) denaturation. Using 10-fold cross-validation test, our method showed a correlation of 0.75 between experimental and predicted values of stability changes, and an accuracy of 82.2% for discriminating the stabilizing and destabilizing mutants.
- Publication
Bioinformatics, 2009, Vol 25, Issue 17, p2181
- ISSN
1367-4803
- Publication type
Academic Journal
- DOI
10.1093/bioinformatics/btp370