We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Extraction of fluorescent cell puncta by adaptive fuzzy segmentation.
- Authors
Pham, Tuan D; Crane, Denis I; Tran, Tuan H; Nguyen, Tam H
- Abstract
The discrimination and measurement of fluorescent-labeled vesicles using microscopic analysis of fixed cells presents a challenge for biologists interested in quantifying the abundance, size and distribution of such vesicles in normal and abnormal cellular situations. In the specific application reported here, we were interested in quantifying changes to the population of a major organelle, the peroxisome, in cells from normal control patients and from patients with a defect in peroxisome biogenesis. In the latter, peroxisomes are present as larger vesicular structures with a more restricted cytoplasmic distribution. Existing image processing methods for extracting fluorescent cell puncta do not provide useful results and therefore, there is a need to develop some new approaches for dealing with such a task effectively.
- Publication
Bioinformatics (Oxford, England), 2004, Vol 20, Issue 14, p2189
- ISSN
1367-4803
- Publication type
Journal Article
- DOI
10.1093/bioinformatics/bth213