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- Title
MethPrimer: designing primers for methylation PCRs.
- Authors
Li, Long-Cheng; Dahiya, Rajvir
- Abstract
DNA methylation is an epigenetic mechanism of gene regulation. Bisulfite- conversion-based PCR methods, such as bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP), remain the most commonly used techniques for methylation mapping. Existing primer design programs developed for standard PCR cannot handle primer design for bisulfite-conversion-based PCRs due to changes in DNA sequence context caused by bisulfite treatment and many special constraints both on the primers and the region to be amplified for such experiments. Therefore, the present study was designed to develop a program for such applications.
- Publication
Bioinformatics (Oxford, England), 2002, Vol 18, Issue 11, p1427
- ISSN
1367-4803
- Publication type
Journal Article
- DOI
10.1093/bioinformatics/18.11.1427