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- Title
AL2CO: calculation of positional conservation in a protein sequence alignment.
- Authors
Pei, J; Grishin, N V
- Abstract
Amino acid sequence alignments are widely used in the analysis of protein structure, function and evolutionary relationships. Proteins within a superfamily usually share the same fold and possess related functions. These structural and functional constraints are reflected in the alignment conservation patterns. Positions of functional and/or structural importance tend to be more conserved. Conserved positions are usually clustered in distinct motifs surrounded by sequence segments of low conservation. Poorly conserved regions might also arise from the imperfections in multiple alignment algorithms and thus indicate possible alignment errors. Quantification of conservation by attributing a conservation index to each aligned position makes motif detection more convenient. Mapping these conservation indices onto a protein spatial structure helps to visualize spatial conservation features of the molecule and to predict functionally and/or structurally important sites. Analysis of conservation indices could be a useful tool in detection of potentially misaligned regions and will aid in improvement of multiple alignments.
- Publication
Bioinformatics (Oxford, England), 2001, Vol 17, Issue 8, p700
- ISSN
1367-4803
- Publication type
Journal Article
- DOI
10.1093/bioinformatics/17.8.700