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- Title
Improving yeast two-hybrid screening systems.
- Authors
Koegl, Manfred; Uetz, Peter
- Abstract
Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein-protein interactions.
- Publication
Briefings in functional genomics & proteomics, 2007, Vol 6, Issue 4, p302
- ISSN
1473-9550
- Publication type
Journal Article
- DOI
10.1093/bfgp/elm035