Title

Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins.

Authors

Kuroiwa, Fu; Suda, Hiraku; Yabuki, Maho; Atsuzawa, Kimie; Yamaguchi, Haruhiko; Toyota, Masatsugu; Kaneko, Yasuko; Yamashita, Satoshi; Takahashi, Seiji; Tozawa, Yuzuru

Abstract

Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.

Subjects

CARRIER proteins; IMMOBILIZED proteins; PROTEIN analysis; FUNCTIONAL analysis; MEMBRANE proteins

Publication

Bioscience, Biotechnology & Biochemistry, 2024, Vol 88, Issue 5, p555

ISSN

0916-8451

Publication type

Academic Journal

DOI

10.1093/bbb/zbae026