We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Plant DNA flow cytometry and estimation of nuclear genome size.
- Authors
Dolezel, Jaroslav; Bartos, Jan
- Abstract
DNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry has become a popular method for ploidy screening, detection of mixoploidy and aneuploidy, cell cycle analysis, assessment of the degree of polysomaty, determination of reproductive pathway, and estimation of absolute DNA amount or genome size. While the former applications are relatively straightforward, estimation of absolute DNA amount requires special attention to possible errors in sample preparation and analysis.
- Publication
Annals of botany, 2005, Vol 95, Issue 1, p99
- ISSN
0305-7364
- Publication type
Journal Article
- DOI
10.1093/aob/mci005