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- Title
Characterization of norovirus-associated traveler's diarrhea.
- Authors
Ajami, N; Koo, H; Darkoh, C; Atmar, R L; Okhuysen, P C; Jiang, Z-D; Flores, J; Dupont, H L
- Abstract
Traveler's diarrhea is the most common medical complaint of international visitors to developing regions. Previous findings suggested that noroviruses (NoVs) are an underappreciated cause of traveler's diarrhea. METHODS. In the present study, we sought to define the presence of NoVs in 320 acute diarrheic stool samples collected from 299 US students who traveled to Guadalajara, Cuernavaca, or Puerto Vallarta, Mexico, during the period from 2007 through 2008. Conventional and quantitative real-time polymerase chain reaction assays were used to detect and determine NoV loads in stool samples. NoV strains were characterized by purification of viral RNA followed by sequencing of the viral capsid protein 1 gene. Sequences were compared using multiple sequence alignment, and phylogenetic trees were generated to evaluate the evolutionary relatedness of the viral strains associated with cases of traveler's diarrhea.
- Publication
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2010, Vol 51, Issue 2, p123
- ISSN
1537-6591
- Publication type
Journal Article
- DOI
10.1086/653530