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- Title
Experimental Protection of Mice against Lethal Staphylococcus aureus Infection by Novel Bacteriophage øMR11.
- Authors
Matsuzak, Shigenobu; Yasuda, Masaharu; Nishikawa, Hiroshi; Kuroda, Masayuki; Ujihara, Takako; Shuin, Taro; Shen, Yuan; Jin, Zhe; Fujimoto, Shigeyoshi; Nasimuzzaman, M.D.; Wakiguchi, Hiroshi; Sugihara, Shigeyoshi; Sugiura, Tetsuro; Koda, Shigeki; Muraoka, Asako; Imai, Shosuke
- Abstract
The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. aureus phages isolated in the study, φMR11 was representatively used for all testing, because its host range was the most broad and it carries no genes for known toxins or antibiotic resistance. Intra-peritoneal injections (8 × 10[SUP8] cells) of S. aureus, including methicillin-resistant bacteria, caused bacteremia and eventual death in mice. In contrast, subsequent intraperitoneal administration of purified φMR11 (MOI⩾0.1) suppressed S. aureus -- induced lethality. This lifesaving effect coincided with the rapid appearance of φMR11 in the circulation, which remained at substantial levels until the bacteria were eradicated. Inoculation with high-dose φMR11 alone produced no adverse effects attributable to the phage. These results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that φMR11 may be a potential prototype for gene-modified, advanced therapeutic S. aureus phages.
- Publication
Journal of Infectious Diseases, 2003, Vol 187, Issue 4, p613
- ISSN
0022-1899
- Publication type
Academic Journal
- DOI
10.1086/374001