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- Title
Herpes simplex virus DNA vaccine efficacy: effect of glycoprotein D plasmid constructs.
- Authors
Strasser, J E; Arnold, R L; Pachuk, C; Higgins, T J; Bernstein, D I
- Abstract
The impact of vaccination with plasmid DNA encoding full-length glycoprotein D (gD) from herpes simplex virus (HSV) type 2 (gD2), secreted gD2, or cytosolic gD2 was evaluated in mice and guinea pigs. Immunization with plasmids encoding full-length gD2 or secreted gD2 produced high antibody levels, whereas immunization with DNA encoding cytosolic gD2 resulted in significantly lower antibody titers in both species (P<.001). Vaccination with DNA encoding full-length or secreted gD2 significantly reduced acute disease in mice and guinea pigs (both P<.001) and subsequent recurrent disease in guinea pigs (P<.05). In guinea pigs, immunization with DNA encoding cytosolic gD2 did not protect from acute or recurrent disease, whereas in mice it did protect, but not as well as DNA encoding full-length or secreted gD2. None of the vaccines resulted in improved virus clearance from the inoculation site, and none significantly reduced recurrent disease when used as a therapeutic vaccine in HSV-2-infected guinea pigs.
- Publication
The Journal of infectious diseases, 2000, Vol 182, Issue 5, p1304
- ISSN
0022-1899
- Publication type
Journal Article
- DOI
10.1086/315878