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- Title
Human immune responses to a novel norwalk virus vaccine delivered in transgenic potatoes.
- Authors
Tacket, C O; Mason, H S; Losonsky, G; Estes, M K; Levine, M M; Arntzen, C J
- Abstract
A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plant-based oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested transgenic potatoes. Twenty-four healthy adult volunteers received 2 or 3 doses of transgenic potato (n=20) or 3 doses of wild-type potato (n=4). Each dose consisted of 150 g of raw, peeled, diced potato that contained 215-751 microgram of NVCP. Nineteen (95%) of 20 volunteers who ingested transgenic potatoes developed significant increases in the numbers of specific IgA antibody-secreting cells. Four (20%) of 20 volunteers developed specific serum IgG, and 6 (30%) of 20 volunteers developed specific stool IgA. Overall, 19 of 20 volunteers developed an immune response of some kind, although the level of serum antibody increases was modest.
- Publication
The Journal of infectious diseases, 2000, Vol 182, Issue 1, p302
- ISSN
0022-1899
- Publication type
Journal Article
- DOI
10.1086/315653