We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Opposite-polarity motors activate one another to trigger cargo transport in live cells.
- Authors
Ally, Shabeen; Larson, Adam G; Barlan, Kari; Rice, Sarah E; Gelfand, Vladimir I
- Abstract
Intracellular transport is typically bidirectional, consisting of a series of back and forth movements. Kinesin-1 and cytoplasmic dynein require each other for bidirectional transport of intracellular cargo along microtubules; i.e., inhibition or depletion of kinesin-1 abolishes dynein-driven cargo transport and vice versa. Using Drosophila melanogaster S2 cells, we demonstrate that replacement of endogenous kinesin-1 or dynein with an unrelated, peroxisome-targeted motor of the same directionality activates peroxisome transport in the opposite direction. However, motility-deficient versions of motors, which retain the ability to bind microtubules and hydrolyze adenosine triphosphate, do not activate peroxisome motility. Thus, any pair of opposite-polarity motors, provided they move along microtubules, can activate one another. These results demonstrate that mechanical interactions between opposite-polarity motors are necessary and sufficient for bidirectional organelle transport in live cells.
- Publication
The Journal of cell biology, 2009, Vol 187, Issue 7, p1071
- ISSN
1540-8140
- Publication type
Journal Article
- DOI
10.1083/jcb.200908075