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- Title
Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes.
- Authors
Lit-Hsin Loo; Hai-Jui Lin; Singh, Dinesh K.; Lyons, Kathleen M.; Altschuler, Steven J.; Wu, Lani F.
- Abstract
Increases in key components of adipogenesis and lipolysis pathways correlate at the population-averaged level during adipogenesis. However, differentiating preadipocytes are highly heterogeneous in cellular and lipid droplet (LD) morphologies, and the degree to which individual cells follow population-averaged trends is unclear. In this study, we analyze the molecular heterogeneity of differentiating 3T3-L1 preadipocytes using immunofluorescence microscopy. Unexpectedly, we only observe a small percentage of cells with high simultaneous expression of markers for adipogenesis (peroxisome proliferator-activated receptor γ [PPARγ], CCAAT/enhancer-binding protein α, and adiponectin) and lipid accumulation (hormone-sensitive lipase, perilipin A, and LDs). Instead, we identify subpopulations of cells with negatively correlated expressions of these readouts. Acute perturbation of adipocyte differentiation with PPARγ agonists, forskolin, and fatty acids induced subpopulation-specific effects, including redistribution of the percentage of cells in observed subpopulations and differential expression levels of PPARγ. Collectively, our results suggested that heterogeneity observed during 3T3-L1 adipogenesis reflects a dynamic mixture of subpopulations with distinct physiological states.
- Publication
Journal of Cell Biology, 2009, Vol 187, Issue 3, p375
- ISSN
0021-9525
- Publication type
Academic Journal
- DOI
10.1083/jcb.200904140