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- Title
Dynamics of inner kinetochore assembly and maintenance in living cells.
- Authors
Hemmerich, Peter; Weidtkamp-Peters, Stefanie; Hoischen, Christian; Schmiedeberg, Lars; Erliandri, Indri; Diekmann, Stephan
- Abstract
To investigate the dynamics of centromere organization, we have assessed the exchange rates of inner centromere proteins (CENPs) by quantitative microscopy throughout the cell cycle in human cells. CENP-A and CENP-I are stable centromere components that are incorporated into centromeres via a "loading-only" mechanism in G1 and S phase, respectively. A subfraction of CENP-H also stays stably bound to centromeres. In contrast, CENP-B, CENP-C, and some CENP-H and hMis12 exhibit distinct and cell cycle-specific centromere binding stabilities, with residence times ranging from seconds to hours. CENP-C and CENP-H are immobilized at centromeres specifically during replication. In mitosis, all inner CENPs become completely immobilized. CENPs are highly mobile throughout bulk chromatin, which is consistent with a binding-diffusion behavior as the mechanism to scan for vacant high-affinity binding sites at centromeres. Our data reveal a wide range of cell cycle-specific assembly plasticity of the centromere that provides both stability through sustained binding of some components and flexibility through dynamic exchange of other components.
- Publication
The Journal of cell biology, 2008, Vol 180, Issue 6, p1101
- ISSN
1540-8140
- Publication type
Journal Article
- DOI
10.1083/jcb.200710052