We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
- Authors
Montembault, Emilie; Dutertre, Stéphanie; Prigent, Claude; Giet, Régis
- Abstract
The spindle checkpoint delays anaphase onset until every chromosome kinetochore has been efficiently captured by the mitotic spindle microtubules. In this study, we report that the human pre-messenger RNA processing 4 (PRP4) protein kinase associates with kinetochores during mitosis. PRP4 depletion by RNA interference induces mitotic acceleration. Moreover, we frequently observe lagging chromatids during anaphase leading to aneuploidy. PRP4-depleted cells do not arrest in mitosis after nocodazole treatment, indicating a spindle assembly checkpoint (SAC) failure. Thus, we find that PRP4 is necessary for recruitment or maintenance of the checkpoint proteins MPS1, MAD1, and MAD2 at the kinetochores. Our data clearly identify PRP4 as a previously unrecognized kinetochore component that is necessary to establish a functional SAC.
- Publication
The Journal of cell biology, 2007, Vol 179, Issue 4, p601
- ISSN
1540-8140
- Publication type
Journal Article
- DOI
10.1083/jcb.200703133