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- Title
Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC<sup>Cdh1</sup>.
- Authors
Marangos, Petros; Verschuren, Emmy W.; Chen, Ruby; Jackson, Peter K.; Carroll, John
- Abstract
Mammalian oocytes are arrested in prophase of the first meiotic division. Progression into the first meiotic division is driven by an increase in the activity of maturation-promoting factor (MPF). In mouse oocytes, we find that early mitotic inhibitor 1 (Emi1), an inhibitor of the anaphase-promoting complex (APC) that is responsible for cyclin B destruction and inactivation of MPF, is present at prophase 1 and undergoes Skp1-Cul1-F-box/βTrCP-mediated destruction immediately after germinal vesicle breakdown (GVBD). Exogenous Emil or the inhibition of Emil destruction in prophase-arrested oocytes leads to a stabilization of cyclin B1-GFP that is sufficient to trigger GVBD. In contrast, the depletion of Emil using morpholino oligonucleotides increases cyclin B1-GFP destruction, resulting in an attenuation of MPF activation and a delay of entry into the first meiotic division. Finally, we show that Emi1-dependent effects on meiosis I require the presence of Cdh1. These observations reveal a novel mechanism for the control of entry into the first meiotic division: an Emi1-dependent inhibition of APC[sup Cdh1].
- Publication
Journal of Cell Biology, 2007, Vol 176, Issue 1, p65
- ISSN
0021-9525
- Publication type
Academic Journal
- DOI
10.1083/jcb.200607070