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- Title
Localization of G<sub>iα</sub> proteins in the centrosomes and at the midbody: implication for their role in cell division.
- Authors
Cho, Hyeseon; Kehrl, John H.
- Abstract
At the plasma membrane, heterotrimeric G proteins act as molecular switches to relay signals from G protein--coupled receptors; however, G[sub α] subunits also have receptor-independent functions at intracellular sites. Regulator of G protein signaling (RGS) 14, which enhances the intrinsic GTPase activity of G[sub iα] proteins, localizes in centrosomes, which suggests the coexpression of G[sub iα]. We show expression of G[sub iα1], G[sub iα2], and G[sub iα3] in the centrosomes and at the midbody. Fluorescence resonance energy transfer analysis confirms a direct interaction between RGS14 and G[sub iα1] in centrosomes. Expression of GTPase-deficient G[sub iα1] results in defective cytokinesis, whereas that of wild-type or GTPase-deficient G[sub iα3] causes prolonged mitosis. Cells treated with pertussis toxin, with reduced expression of G[sub iα1], G[sub iα2], and G[sub iα3] or with decreased expression of RGS14 also exhibit cytokinesis defects. These results suggest that G[sub iα] proteins and their regulators at these sites may play essential roles during mammalian cell division.
- Publication
Journal of Cell Biology, 2007, Vol 178, Issue 2, p245
- ISSN
0021-9525
- Publication type
Academic Journal
- DOI
10.1083/jcb.200604114