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- Title
The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3, 5P<sub>2</sub> synthesis and turnover.
- Authors
Duex, Jason E.; Tang, Fusheng; Weisman, Lois S.
- Abstract
Phosphoinositide-signaling lipids function in diverse cellular pathways. Dynamic changes in the levels of these signaling lipids regulate multiple processes. In particular, when Saccharomyces cerevisiae cells are exposed to hyperosmotic shock, PI3,5P[sub 2] (phosphatidylinositol [PI] 3,5-bisphosphate) levels transiently increase 20-fold. This causes the vacuole to undergo multiple acute changes. Control of PI3,5P[sub 2] levels occurs through regulation of both its synthesis and turnover. Synthesis is catalyzed by the PI3P 5-kinase Fab1p, and turnover is catalyzed by the PI3,5P[sub 2] 5-phosphatase Fig4p. In this study, we show that two putative Fab1p activators, Vac7p and Vac14p, independently regulate Fab1p activity. Although Vac7p only regulates Fab1p, surprisingly, we find that Vac14 regulates both Fab1p and Fig4p. Moreover, Fig4p itself functions in both PI3,5P[sub 2] synthesis and turnover. In both the absence and presence of Vac7p, the Vac14p-Fig4p complex controls the hyperosmotic shock-induced increase in PI3,5P[sub 2] levels. These findings suggest that the dynamic changes in PI3,5P[sub 2] are controlled through a tight coupling of synthesis and turnover.
- Publication
Journal of Cell Biology, 2006, Vol 172, Issue 5, p683
- ISSN
0021-9525
- Publication type
Academic Journal
- DOI
10.1083/jcb.200512105