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- Title
Molecular constituents of neuronal AMPA receptors.
- Authors
Fukata, Yuko; Tzingounis, Anastassios V; Trinidad, Jonathan C; Fukata, Masaki; Burlingame, Alma L; Nicoll, Roger A; Bredt, David S
- Abstract
Dynamic regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) underlies aspects of synaptic plasticity. Although numerous AMPAR-interacting proteins have been identified, their quantitative and relative contributions to native AMPAR complexes remain unclear. Here, we quantitated protein interactions with neuronal AMPARs by immunoprecipitation from brain extracts. We found that stargazin-like transmembrane AMPAR regulatory proteins (TARPs) copurified with neuronal AMPARs, but we found negligible binding to GRIP, PICK1, NSF, or SAP-97. To facilitate purification of neuronal AMPAR complexes, we generated a transgenic mouse expressing an epitope-tagged GluR2 subunit of AMPARs. Taking advantage of this powerful new tool, we isolated two populations of GluR2 containing AMPARs: an immature complex with the endoplasmic reticulum chaperone immunoglobulin-binding protein and a mature complex containing GluR1, TARPs, and PSD-95. These studies establish TARPs as the auxiliary components of neuronal AMPARs.
- Publication
The Journal of cell biology, 2005, Vol 169, Issue 3, p399
- ISSN
0021-9525
- Publication type
Journal Article
- DOI
10.1083/jcb.200501121