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- Title
Novel way of capping mRNA trimer and studies of its interaction with human nuclear cap-binding complex.
- Authors
Worch, Remigiusz; Stepinski, Janusz; Niedzwiecka, Anna; Jankowska-Anyszka, Marzena; Mazza, Catherine; Cusack, Stephen; Stolarski, Ryszard; Darzynkiewicz, Edward
- Abstract
Binding of mRNA 5' cap by the nuclear cap-binding complex (CBC) is crucial for a wide variety of mRNA metabolic events. The interaction involving the CBP20 subunit of CBC is mediated by numerous hydrogen bonds and by stacking of the tyrosine sidechains with two first bases of the capped mRNA. To examine a possible role of a longer mRNA chain in the CBC-cap recognition, we have synthesized an mRNA tetramer using a novel way of capping an RNA trimer and determined its affinity for CBC by fluorescence titration.
- Publication
Nucleosides, nucleotides & nucleic acids, 2005, Vol 24, Issue 5-7, p1131
- ISSN
1525-7770
- Publication type
Journal Article
- DOI
10.1081/ncn-200061898