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- Title
The use and evaluation of 2 + 2 photoaddition in immobilization of oligonucleotides on a three dimensional hydrogel matrix.
- Authors
Elghanian, R; Xu, Y; McGowen, J; Seithoff, M; Liu, C G; Winick, J; Fuller, N; Ramakrishnan, R; Beuhler, A; Johnson, T; Mazumder, A; Brush, C K
- Abstract
Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2 + 2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.
- Publication
Nucleosides, nucleotides & nucleic acids, 2001, Vol 20, Issue 4-7, p1371
- ISSN
1525-7770
- Publication type
Journal Article
- DOI
10.1081/NCN-100002558