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- Title
Projection structure of full length connexin 43 by electron cryo-crystallography.
- Authors
Cheng, Anchi; Schweissinger, Daniel; Dawood, Farah; Kumar, Nalin; Yeager, Mark
- Abstract
We previously used electron cryo-crystallography to determine the three-dimensional structure of recombinant gap junction channels formed by a C-terminal truncation mutant of Cx43 (11). The dodecameric channel is formed by the end-to-end docking of two hexameric connexons, each comprised of 24 transmembrane alpha-helices. We have now generated two-dimensional crystals of the recombinant, full-length channel, as well as crystals in which the C-tail has been completely removed by trypsin digestion. Projection density maps at 7.5 A resolution closely resemble our previous analysis of the C-terminal truncation mutant (9). A difference map between the full length and trypsin-treated channels suggests that there are small but significant shifts in protein density upon removal of the C-tail.
- Publication
Cell communication & adhesion, 2003, Vol 10, Issue 4-6, p187
- ISSN
1541-9061
- Publication type
Journal Article
- DOI
10.1080/cac.10.4-6.187.191