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- Title
Simultaneous estimation of hydroxychavicol and chlorogenic acid from Piper betel L. through RP-HPLC.
- Authors
Maity, Niladri; Nema, Neelesh K; Sellamuthu, Mythies K; Sarkar, Birendra K; Mukherjee, Pulok K
- Abstract
A RP-HPLC method was developed (λ (max) =280) to quantify hydroxychavicol and chlorogenic acid in Piper betel Linn. The method was validated for linearity, limit of detection (LOD=3:1σ/S), limit of quantification (LOQ=10:1σ/S), precision, accuracy and ruggedness. The response was linear with good correlation between concentration and mean peak area through a coefficient of determinants (r (2)) of 0.9940, y=1.98e +004x +5.19e +004 and 0.9945, y=2.76e+004x+1.40e+005 with LOD 1.6 µg mL(-1), 1.0 µg mL(-1) and LOQ 5.0 µg mL(-1) and 3.0 µg mL(-1), respectively, for hydroxychavicol (28.56% w/w) and chlorogenic acid (0.40% w/w). The %RSD of precision and recovery of hydroxychavicol and chlorogenic acid were <2.0%. The proposed method was simple, accurate, specific, precise and reproducible.
- Publication
Natural product research, 2012, Vol 26, Issue 20, p1939
- ISSN
1478-6427
- Publication type
Journal Article
- DOI
10.1080/14786419.2011.619189