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- Title
Preferential Binding of Leukocytes to the Endothelial Junction Region in Venules In Situ.
- Authors
Wojciechowski, Joel C.; Sarelius, Ingrid H.
- Abstract
Objective: To determine whether leukocytes show a preference to firmly adhere to the endothelium near the endothelial cell (EC) junction in their native environment, i.e., blood-perfused venules in situ. Methods: Intravital confocal microscopy was used to determine the positions of firmly adherent leukocytes with respect to EC junctions in cremaster muscle venules in anesthetized mice. EC area and EC junction locations were identified using immunofluorescent labeling of platelet–endothelial cell adhesion molecule-1 (PECAM-1), an endothelial junction protein. Leukocytes were identified using transillumination through the same optical path. Results: Seventy-five percent of firmly adherent leukocytes overlapped an EC junction (the average distance to the nearest EC junction was 2.4 ± 0.1 µ m, n = 263). This distance was less in smaller diameter venules, hence the percentage of adherent leukocytes that overlapped an endothelial junction was larger. EC shape and size varied with venular diameter: in smaller venules (30–49 µ m diameter), ECs had significantly less area (316 ± 19 µ m 2 ) and were narrower (12.5 ± 0.4 µ m) than in larger (70–89 µ m diameter) venules, which had 614 ± 28 µ m 2 area and 17.8 ± 0.5 µ m width, respectively ( p <> <CONTINFO> <DOI>10.1080/10739680590934763</DOI> <PII>X256Q5H6866NK040</PII> <PPCT> <PPF>349</PPF> <PPL>359</PPL> </CONTINFO> <ISSUE> <PINFO> <PNM>Taylor & Francis</PNM> </PINFO> <JINFO> <JCODE>UMIC</JCODE> <JTL>Microcirculation</JTL> <ISSN>1073-9688</ISSN> <URL>http://taylorandfrancis.metapress.com/link.asp?target=journal&id=111039</URL> </JINFO> <PUBINFO> <VID>12</VID> <IID>4</IID> <CD>Number 4/June 2005</CD> </PUBINFO> <FULLTEXT> <FILENAME>X256Q5H6866NK040.pdf</FILENAME> <URL>http://taylorandfrancis.metapress.com/link.asp?target=contribution&id=X256Q5H6866NK040</URL> </FULLTEXT> <URL>http://taylorandfrancis.metapress.com/link.asp?target=issue&id=LW60816U5W44</URL> </ISSUE> <AUG> <AU> <FNMS>JOEL</FNMS> <SNM>WOJCIECHOWSKI</SNM> <ORF> <AU> <FNMS>INGRID</FNMS> <SNM>SARELIUS</SNM> <ORF> <AFF> <OID> Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York, USA <AFF> <OID> Department of Pharmacology and Physiology, University of Rochester, Rochester, New York, USA </AUG> <KWDG> <KWD>endothelial cell junctions</KWD> <KWD>leukocyte diapedesis</KWD> <KWD>leukocyte motility</KWD> </KWDG> </REFG>
- Publication
Microcirculation, 2005, Vol 12, Issue 4, p1
- ISSN
1073-9688
- Publication type
Academic Journal
- DOI
10.1080/10739680590934763