We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phosphate increases mitochondrial reactive oxygen species release.
- Authors
Oliveira, Graciele A; Kowaltowski, Alicia J
- Abstract
The effects of inorganic phosphate (Pi), the main intracellular membrane permeable anion capable of altering mitochondrial pH gradients (Delta pH), were measured on mitochondrial H2O2 release. As expected, Pi decreased Delta pH and increased the electric membrane potential (Delta Psi). Mitochondrial H2O2 release was stimulated by Pi and also by its structural analogue arsenate. However, acetate, another membrane-permeable anion, did not stimulate mitochondrial H2O2 release. The stimulatory effect promoted by Pi was prevented by CCCP, which decreases transport of Pi across the inner mitochondrial membrane, indicating that Pi must be in the mitochondrial matrix to stimulate H2O2 release. In conclusion, we found that Pi and arsenate stimulate mitochondrial reactive oxygen release, an effect that may contribute towards oxidative stress under conditions such as ischemia/reperfusion, in which high-energy phosphate bonds are hydrolyzed.
- Publication
Free radical research, 2004, Vol 38, Issue 10, p1113
- ISSN
1071-5762
- Publication type
Journal Article
- DOI
10.1080/10715760400009258