We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice.
- Authors
Yamamoto, Yoritsuna; Yamada, Satoko; Kodera, Tsutomu; Hara, Akemi; Motoyoshi, Kazuo; Tanaka, Yuji; Nagaoka, Tadahiro; Seno, Masaharu; Kojima, Itaru
- Abstract
Previous studies have shown the efficacy of betacellulin (BTC) to promote beta-cell regeneration. Because of its short half-life, however, the effect of BTC may have been underestimated. This study was conducted to assess the effect of continuous administration of BTC on beta-cell regeneration. Adenovirus vectors encoding proBTC (Ad-proBTC) and mature BTC (Ad-mBTC) were prepared, and the efficacy of secretion of BTC was compared in AML12 hepatocytes. When AML12 cells were infected with Ad-proBTC or Ad-mBTC, cells infected with Ad-mBTC secreted considerably larger amount of BTC. We then infused Ad-mBTC into the mouse tail vein. Expression of BTC was detected in the liver for at least 21 days, and serum BTC was maintained at approximately 1 ng/ml for 7 days. When Ad-mBTC was infused immediately after administration of STZ (170 mg/kg), elevation of the plasma glucose induced by STZ was markedly inhibited, and the plasma glucose concentration remained at less than 200 mg/dl for 21 days. The insulin content and the beta-cell mass were significantly increased in Ad-mBTC-infused mice. These results indicate that continuous administration of BTC is quite effective in promoting regeneration of beta-cells.
- Publication
Growth factors (Chur, Switzerland), 2008, Vol 26, Issue 4, p173
- ISSN
0897-7194
- Publication type
Journal Article
- DOI
10.1080/08977190802136854