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- Title
A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays.
- Authors
Fei, Y Y; Landry, J P; Sun, Y S; Zhu, X D; Luo, J T; Wang, X B; Lam, K S
- Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
- Publication
The Review of scientific instruments, 2008, Vol 79, Issue 1, p013708
- ISSN
0034-6748
- Publication type
Journal Article
- DOI
10.1063/1.2830286