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- Title
Binding of the GABA<sub>A</sub> Receptor-Associated Protein (GABARAP) to Microtubules and Microfilaments Suggests Involvement of the Cytoskeleton in GABARAPGABA<sub>A</sub> Receptor Interaction.
- Authors
Wang, Hongbing; Olsen, Richard W
- Abstract
GABAA receptor-associated protein (GABARAP) was isolated on the basis of its interaction with the γ2 subunit of GABAA receptors. It has sequence similarity to light chain 3 (LC3) of microtubule-associated proteins 1A and 1B. This suggests that GABARAP may link GABAA receptors to the cytoskeleton. GABARAP associates with tubulin in vitro. However, little is known about the mechanism for the interaction, and it is not clear whether the interaction occurs in vivo. Here, we report that GABARAP interacts directly with both tubulin and microtubules in a salt-sensitive manner, indicating the association is mediated by ionic interactions. GABARAP coimmunoprecipitates with tubulin and associates with both microtubules and microfilaments in intact cells. The cellular distribution is altered by treatment with taxol, nocodazole, and cytochalasin D. The tubulin binding domain was located at the N terminus of GABARAP by using synthetic peptides and deletion constructs and is marked by a specific arrangement of basic amino acids. The interaction between GABARAP and actin might be mediated by other proteins. These results demonstrate the GABARAP interacts with the cytoskeleton both in vitro and in cells and suggest a role of GABARAP in the interaction between GABAA receptors and the cytoskeleton. Such interactions are presumably needed for receptor trafficking, anchoring, and/or synaptic clustering. The structural arrangement of the basic amino acids present in the tubulin binding domain of GABARAP may aid in recognition of the potential of tubulin binding activity in other known proteins.
- Publication
Journal of Neurochemistry, 2000, Vol 75, Issue 2, p644
- ISSN
0022-3042
- Publication type
Academic Journal
- DOI
10.1046/j.1471-4159.2000.0750644.x