Birch PR-10c is induced by factors causing oxidative stress but appears not to confer tolerance to these agents.

Koistinen, Kaisa M.; Hassinen, Viivi H.; Gynther, Petra A. M.; Lehesranta, Satu J.; Keinänen, Sirpa I.; Kokko, Harri I.; Oksanen, Elina J.; Tervahauta, Arja I.; Auriola, Seppo; Kärenlampi, Sirpa O.

Summary • Expression of all known and newly found pathogenesis-related PR-10 proteins (PR-10a, b, c, d, e) was analysed from Cu-sensitive and –tolerant birch clones to find out whether they follow the same expression pattern. The relationship of PR-10 proteins, particularly PR-10c, to oxidative stress caused by metals or ozone was studied in tolerant and sensitive birch clones to find out possible linkages to tolerance. • Antibody developed to PR-10c was used in Western blot analysis. Other PR-10 proteins were studied with two-dimensional electrophoresis and mass spectrometry. Metal-sensitive yeasts were transformed with PR-10c. • Two new members of PR-10 family, PR-10d and PR-10e, were found. Various PR-10 proteins showed different expression patterns. The amount of PR-10c increased with increasing soil metal concentrations but was, in general, more prominent in Cu-sensitive than in Cu-tolerant clones. PR-10c did not alter metal tolerance in metal-sensitive yeasts. • The PR-10c protein appears not to confer metal- or ozone-tolerance in birch. However, this does not exclude the possibility that it is involved in the tolerance or sensitivity mechanism in an indirect manner.

BIRCH; PROTEINS; PLANT ecology; MASS spectrometry

New Phytologist, 2002, Vol 155, Issue 3, p381

0028-646X

Academic Journal

10.1046/j.1469-8137.2002.00481.x