We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic and molecular evidence that the Pseudomonas syringae type III effector protein AvrRpt2 is a cysteine protease.
- Authors
Axtell, Michael J; Chisholm, Stephen T; Dahlbeck, Douglas; Staskawicz, Brian J
- Abstract
Upon delivery to the plant cell during infection, the Pseudomonas syringae effector protein AvrRpt2 undergoes proteolytic processing, enhances pathogen virulence and causes the elimination of the Arabidopsis RIN4 protein. A structure-prediction method was employed in order to investigate possible biochemical functions of AvrRpt2. Results of a secondary structure prediction algorithm suggest that the functional C-terminal portion of AvrRpt2 is a cysteine protease. Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2-specific resistance response. These data indicate that AvrRpt2 is most likely a sequence divergent cysteine protease whose activity is required for elimination of RIN4 during infection.
- Publication
Molecular microbiology, 2003, Vol 49, Issue 6, p1537
- ISSN
0950-382X
- Publication type
Journal Article
- DOI
10.1046/j.1365-2958.2003.03666.x