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- Title
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis σ <sup>W</sup> and σ <sup>M</sup> regulons.
- Authors
Cao, Min; Wang, Tao; Ye, Rick; Helmann, John D.
- Abstract
Summary Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma factors. The σ W regulon includes functions involved in detoxification and protection against antimicrobials, whereas σ M is essential for growth at high salt concentrations. We now report that antibiotics that inhibit cell wall biosynthesis induce both σ W and σ M regulons as monitored using DNA microarrays. Induction of selected σ W -dependent genes was confirmed using lacZ reporter fusions and Northern blot analysis. The ability of vancomycin to induce the σ W regulon is dependent on both σ W and the cognate anti- σ , RsiW, but is independent of the transition state regulator AbrB. These results suggest that the membrane-localized RsiW anti- σ W factor mediates the transcriptional response to cell wall stress. Our findings are consistent with the idea that one function of ECF σ factors is to coordinate antibiosis stress responses and cell envelope homeostasis.
- Publication
Molecular Microbiology, 2002, Vol 45, Issue 5, p1267
- ISSN
0950-382X
- Publication type
Academic Journal
- DOI
10.1046/j.1365-2958.2002.03050.x