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- Title
A molecular mechanism for the repression of transcription by the H-NS protein.
- Authors
Rimsky, S; Zuber, F; Buckle, M; Buc, H
- Abstract
The H-NS protein is a major component of the bacterial nucleoid and plays a crucial role in the global gene regulation of enteric bacteria. Although H-NS does not exhibit a high DNA sequence specificity, a number of H-NS-responsive promoters have been shown to contain regions of intrinsic DNA curvature located either upstream or downstream of the transcription start point. We have studied H-NS binding to DNA and in vitro transcriptional regulation by H-NS at several synthetic promoters with or without curved sequences inserted upstream of the Pribnow box. We show how such inserts determine the final organization of H-NS-containing nucleoprotein complexes and how this affects transcription. We refine a two-step mechanism for the constitution of H-NS assemblies that are efficient in regulation.
- Publication
Molecular microbiology, 2001, Vol 42, Issue 5, p1311
- ISSN
0950-382X
- Publication type
Journal Article
- DOI
10.1046/j.1365-2958.2001.02706.x