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- Title
Adaptive correction of depth-induced aberrations in multiphoton scanning microscopy using a deformable mirror.
- Authors
Sherman, L; Ye, J Y; Albert, O; Norris, T B
- Abstract
We demonstrate adaptive aberration correction for depth-induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two-photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40x/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.
- Publication
Journal of microscopy, 2002, Vol 206, Issue Pt 1, p65
- ISSN
0022-2720
- Publication type
Journal Article
- DOI
10.1046/j.1365-2818.2002.01004.x