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- Title
piggyBac-mediated germline transformation in the beetle Tribolium castaneum.
- Authors
Lorenzen, M D; Berghammer, A J; Brown, S J; Denell, R E; Klingler, M; Beeman, R W
- Abstract
The lepidopteran transposable element piggyBac can mediate germline insertions in at least four insect orders. It therefore shows promise as a broad-spectrum transformation vector, but applications such as enhancer trapping and transposon-tag mutagenesis are still lacking. We created, cloned, sequenced and genetically mapped a set of piggyBac insertions in the red flour beetle, Tribolium castaneum. Transpositions were precise, and specifically targeted the canonical TTAA recognition sequence. We detected several novel reporter-expression domains, indicating that piggyBac could be used to identify enhancer regions. We also demonstrated that a primary insertion of a non-autonomous element can be efficiently remobilized to non-homologous chromosomes by injection of an immobile helper element into embryos harbouring the primary insertion. These developments suggest potential for more sophisticated methods of piggyBac-mediated genome manipulation.
- Publication
Insect molecular biology, 2003, Vol 12, Issue 5, p433
- ISSN
0962-1075
- Publication type
Journal Article
- DOI
10.1046/j.1365-2583.2003.00427.x