We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Targeted disruption of a pupal hemocyte protein of Sarcophaga by RNA interference.
- Authors
Nishikawa, T; Natori, S
- Abstract
Previously, we purified a transmembrane protein with a molecular mass of 120 kDa (p120) that is exclusively expressed in pupal hemocytes of Sarcophaga. In this study, we demonstrated that double-stranded RNA (dsRNA) injected into the larval body cavity effectively inhibited the expression of p120 in pupal hemocytes. Thus, RNA interference (RNAi) was found to be a useful technique for creating pupal hemocytes with a loss-of-function of a specific protein. The p120-less pupal hemocytes generated by RNAi were found to have lost the ability to take up acetylated low density lipoprotein, indicating that p120 is a scavenger receptor specifically expressed on the surface of pupal hemocytes.
- Publication
European journal of biochemistry, 2001, Vol 268, Issue 20, p5295
- ISSN
0014-2956
- Publication type
Journal Article
- DOI
10.1046/j.0014-2956.2001.02461.x