We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhanced methyltransferase activity of SMYD3 by the cleavage of its N-terminal region in human cancer cells.
- Authors
Silva, F Pittella; Hamamoto, R; Kunizaki, M; Tsuge, M; Nakamura, Y; Furukawa, Y
- Abstract
Histone methylation is involved in the regulation of gene expression and DNA replication through alteration of chromatin structure. We earlier showed that SMYD3, a histone H3-lysine 4-specific methyltransferase, is frequently upregulated in human colorectal, liver and breast cancer compared to their matched non-cancerous cells, and that its activity is associated with the growth of these tumors. In the present study, we found that human cancer cells express both the full-length and a cleaved form of SMYD3 protein. Amino acid sequence analysis uncovered that the cleaved form lacks the 34 amino acids in the N-terminal region of the full-length protein. Interestingly, the cleaved protein and mutant protein containing substitutions at glycines 15 and 17, two highly conserved amino acids in the N-terminal region, revealed a higher histone methyltransferase (HMTase) activity compared to the full-length protein. Furthermore, the N-terminal region is responsible for the association with heat shock protein 90alpha (HSP90alpha). These data indicate that the N-terminal region plays an important role for the regulation of its methyltransferase activity and suggest that a structural change of the protein through the cleavage of the region or interaction with HSP90alpha may be involved in the modulation. These findings may help for a better understanding of the mechanisms that modulate the HMTase activity of SMYD3, and contribute to the development of novel anticancer drugs targeting SMYD3 methyltransferase activity.
- Publication
Oncogene, 2008, Vol 27, Issue 19, p2686
- ISSN
1476-5594
- Publication type
Journal Article
- DOI
10.1038/sj.onc.1210929