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- Title
Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer.
- Authors
Kruzelock, R P; Cuevas, B D; Wiener, J R; Xu, F J; Yu, Y; Cabeza-Arvelaiz, Y; Pershouse, M; Lovell, M M; Killary, A M; Mills, G B; Bast, R C, Jr
- Abstract
The identity of many tumor suppressor genes important in epithelial ovarian cancer tumorigenesis remains unknown. In an effort to localize a novel tumor suppressor on chromosome 22, a psv2neo tagged human chromosome 22 was transferred into the malignant epithelial ovarian cancer cell line, SKOv-3, by microcell-mediated chromosome transfer. Complete suppression of the transformed phenotype was observed in 16 of 18 individual microcell hybrid clones as evidenced by the complete abrogation of cell growth under anchorage-independent conditions. In vitro doubling times were also dramatically reduced, as was the ability to form subcutaneous tumors in CD1 nu/nu mice. Only one polymorphic marker, D22S429, segregated with decreased transformation and tumorigenic potential, suggesting that an unrecognized tumor suppressor may localize to chromosome 22q11-q12. These data provide functional support for the presence of a novel tumor suppressor locus (or loci) on chromosome 22 that is important in ovarian cancer tumorigenesis.
- Publication
Oncogene, 2000, Vol 19, Issue 54, p6277
- ISSN
0950-9232
- Publication type
Journal Article
- DOI
10.1038/sj.onc.1204013